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Measuring the effects of fractionated radiation therapy in a 3D prostate cancer model system using SERS nanosensors.
Camus, Victoria L; Stewart, Grant D; Nailon, William H; McLaren, Duncan B; Campbell, Colin J.
Affiliation
  • Camus VL; School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, UK. colin.campbell@ed.ac.uk.
  • Nailon WH; Edinburgh Radiation Research Collaborative, Oncology Physics, Western General Hospital, Edinburgh EH4 2U, UK.
  • McLaren DB; Edinburgh Radiation Research Collaborative, Edinburgh Cancer Centre, Western General Hospital, Edinburgh EH4 2U, UK.
  • Campbell CJ; School of Chemistry, University of Edinburgh, Edinburgh, EH9 3FJ, UK. colin.campbell@ed.ac.uk.
Analyst ; 141(17): 5056-61, 2016 08 15.
Article in En | MEDLINE | ID: mdl-27310732
Multicellular tumour spheroids (MTS) are three-dimensional cell cultures that possess their own microenvironments and provide a more meaningful model of tumour biology than monolayer cultures. As a result, MTS are becoming increasingly used as tumor models when measuring the efficiency of therapies. Monitoring the viability of live MTS is complicated by their 3D nature and conventional approaches such as fluorescence often require fixation and sectioning. In this paper we detail the use of Surface Enhanced Raman Spectroscopy (SERS) to measure the viability of MTS grown from prostate cancer (PC3) cells. Our results show that we can monitor loss of viability by measuring pH and redox potential in MTS and furthermore we demonstrate that SERS can be used to measure the effects of fractionation of a dose of radiotherapy in a way that has potential to inform treatment planning.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Spectrum Analysis, Raman / Spheroids, Cellular / Dose Fractionation, Radiation Limits: Humans / Male Language: En Journal: Analyst Year: 2016 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Spectrum Analysis, Raman / Spheroids, Cellular / Dose Fractionation, Radiation Limits: Humans / Male Language: En Journal: Analyst Year: 2016 Document type: Article Country of publication: